cell counts 10 4 cells cdna was synthesized using the lunascript rt mastermix Search Results


eight-chamber slide glass  (Inter-Med Inc)
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Inter-Med Inc eight-chamber slide glass
Eight Chamber Slide Glass, supplied by Inter-Med Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analyzed by flow cytometry  (Becton Dickinson)
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Becton Dickinson analyzed by flow cytometry
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mattek glass bottom dish  (Becton Dickinson)
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Becton Dickinson mattek glass bottom dish
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svec4-10 cells  (Lonza)
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trizol ls  (Thermo Fisher)
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cell proliferation  (Dojindo Labs)
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Dojindo Labs cell proliferation
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cell migration assay insert  (Becton Dickinson)
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Becton Dickinson cell migration assay insert
Cell Migration Assay Insert, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matrigel  (Becton Dickinson)
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Becton Dickinson matrigel
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matrigel-coated membrane  (Corning Life Sciences)
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Corning Life Sciences matrigel-coated membrane
Functional analysis of EMT using wound healing and <t>Matrigel</t> invasion assays <t>following</t> <t>TGF-β</t> treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).
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cell quest pro software  (Becton Dickinson)
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Becton Dickinson cell quest pro software
Functional analysis of EMT using wound healing and <t>Matrigel</t> invasion assays <t>following</t> <t>TGF-β</t> treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).
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cd4 t cell isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec cd4 t cell isolation kit
Functional analysis of EMT using wound healing and <t>Matrigel</t> invasion assays <t>following</t> <t>TGF-β</t> treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).
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rpmi-1640 medium  (Corning Life Sciences)
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Corning Life Sciences rpmi-1640 medium
Functional analysis of EMT using wound healing and <t>Matrigel</t> invasion assays <t>following</t> <t>TGF-β</t> treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).
Rpmi 1640 Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Functional analysis of EMT using wound healing and Matrigel invasion assays following TGF-β treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).

Journal: Scientific Reports

Article Title: TGF-β induced EMT and stemness characteristics are associated with epigenetic regulation in lung cancer

doi: 10.1038/s41598-020-67325-7

Figure Lengend Snippet: Functional analysis of EMT using wound healing and Matrigel invasion assays following TGF-β treatment. ( A ) Although the growth rate of lung cells treated with TGF-β was different, cell migration toward the center of scratched area was higher in lung cells induced by TGF-β. ( B ) Transwell invasion in BEAS-2B, A549, H292, H226, and H460 cells following TGF-β treatment was enhanced. Data are presented as mean ± SE (n = 4, * P < 0.05).

Article Snippet: Migrated cells were cultured in serum-free medium with or without 10 ng/mL TGF-β for 72 h. After treatment with TGF-β for 72 h, 2.5 × 10 4 cells were seeded onto Matrigel-coated membrane with Falcon Cell Culture Inserts in 24-well plates (pore size, 8 μm; Corning Inc., Corning, NY, USA) containing serum-free medium.

Techniques: Functional Assay, Migration